| |
|
|
| Registros recuperados : 7 | |
| 2. |  | CABRERA, A.; BERNÁ, L.; FRESIA, P.; SILVEIRA, C.S.; MACÍAS-RIOSECO, M.; PRITSCH,O.; RIET-CORREA, F.; GIANNITTI, F.; FRANCIA, M.E.; REBOLLO, C. Generación de nuevas herramientas para el control de Neospora caninum a partir de un enfoque epidemiológico y genómico. [Resumen] In: REDBIO; INIA (Instituto Nacional de Investigación Agropecuaria); REDBIO Argentina. X Encuentro Latinoamericano y del Caribe de Biotecnología Agropecuaria y XI Simposio Redbio Argentina. Libro de Resúmenes. Montevideo 12 - 15 Noviembre 2019. Montevideo (UY): INIA, 2019. p. 148. (INIA Serie Técnica; 253).| Biblioteca(s): INIA La Estanzuela. |
|    |
| 3. | | MANISSE, N.; CABRERA, A.; HERNÁN, J.; COSSE, M.; GIANNITTI, F.; FRANCIA, M.E.; GONZÁLEZ, T.; IRIARTE, A.; RIET-CORREA, F.; ROBELLO, C.; GONZÁLEZ, S. Molecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes: a contribution to their molecular epidemiology. Molecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w Article history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de
Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación...| Biblioteca(s): INIA La Estanzuela. |
|  |
| 4. | | MACÍAS-RIOSECO, M.; SILVEIRA, C.S.; FRAGA, M.; CASAUX, M.L.; CABRERA, A.; FRANCIA, M.E.; REBOLLO, C.; MAYA, L.; ZARANTONELLI, L.; SUANES, A.; COLINA, R.; BUSCHIAZZO, A.; GIANNITTI, F.; RIET-CORREA, F. Causes of abortion in dairy cows in Uruguay. [Causas de aborto em bovinos de leite no Uruguai]. Pesquisa Veterinaria Brasileira, 1 May 2020, Volume 40, Issue 5, Pages 325-332. OPEN ACCESS. DOI: https://doi.org/10.1590/1678-5150-pvb-6550 Article history: Received on December 3, 2019./Accepted for publication on December 26, 2019. This research was financially supported by Agencia Nacional de Investigación e Innovación (ANII), grant FSSA_X_2014_1_105696. The authors thank...| Biblioteca(s): INIA La Estanzuela. |
| |
| 5. | | CABRERA, A.; FRESIA, P.; BERNÁ, L.; SILVEIRA, C.S.; MACÍAS-RIOSECO, M.; AREVALO, A.P.; CRISPO, M.; PRITSCH, O.; RIET-CORREA, F.; GIANNITTI, F.; GIANNITTI, F.; FRANCIA, M.E.; ROBELLO, C. Isolation and molecular characterization of four novel Neospora caninum strains. Genetics, Evolution, and Phylogeny - Short Communication. Parasitology Research, 1 December 2019, Volume 118, Issue 12, Pages 3535-3542. Doi: 10.1007/s00436-019-06474-9 Article history: Received: 11 April 2019 / Accepted: 24 September 2019 / Published online: 7 November 2019.
Funding Sponsor: Agencia Nacional de Investigación e Innovación (ANII).
Funding Text: This project was funded by grant...| Biblioteca(s): INIA Las Brujas. |
| |
| 6. | | DORSCH, M.; FRANCIA, M.E.; TANA, L.R.; GONZÁLEZ, F.C.; CABRERA, A.; CALLEROS, L.; SANGUINETTI, M.; BARCELLOS, M.; ZARANTONELLI, L; CIUFFO, C.; MAYA, L.; CASTELLS, M.; MIRAZO, S.; SILVEIRA, C.S.; RABAZA, A.; CAFFARENA, D.; DONCEL, B.; ARÁOZ, V.; MATTO, C.; RMENDANO, J.I.; SALADA, S.; FRAGA, M.; FIERRO, S.; GIANNITTI, F. Diagnostic investigation of 100 cases of abortion in sheep in Uruguay: 2015-2021. Frontiers in Veterinary Science, 2022; i. 9:904786. OPEN ACCESS. Doi: https://doi.org/10.3389/fvets.2022.904786. Article history: Received: 25 March 2022/Accepted: 13 April 2022/Published: 19 May 2022.| Biblioteca(s): INIA La Estanzuela. |
| |
| 7. | | GIANNITTI, F.; FRANCIA, M.E.; TANA, L.; GONZÁLEZ, F.; CABRERA, A.; CALLEROS, L.; SANGUINETTI, M.; BARCELLOS, M.; ZARANTONELLI, L.; CIUFFO, C.; MAYA, L.; CASTELLS, M.; COLINA, R.; MIRAZO, S.; SILVEIRA, C.S.; RABAZA, A.; CAFFARENA, D.; DONCEL, B.; ARÁOZ, V.; MATTO, C.; ARMENDANO, J.; SALADA, S.; SARAVIA, A.; CASAUX, M.L.; SCHILD, C.; PERDOMO, Y.; FRAGA, M.; FIERRO, S.; DORSCH, M. Causas de aborto en ovinos de Uruguay: 100 casos, 2015-2021. Producción Animal. Revista INIA Uruguay, Setiembre 2022, no.70, p.18-22. (Revista INIA; 70). Agradecimientos: A todos los productores y técnicos del SUL y actividad liberal que enviaron casos al laboratorio y a los funcionarios de INIA, Institut Pasteur de Montevideo, Udelar y DILAVE que contribuyeron con los análisis de...| Biblioteca(s): INIA Las Brujas. |
| |
| Registros recuperados : 7 | |
|
|
|
 | Acceso al texto completo restringido a Biblioteca INIA La Estanzuela. Por información adicional contacte bib_le@inia.org.uy. |
|
Registro completo
|
|
Biblioteca (s) : |
INIA La Estanzuela. |
|
Fecha actual : |
07/07/2021 |
|
Actualizado : |
27/06/2022 |
|
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
|
Circulación / Nivel : |
Internacional - -- |
|
Autor : |
MANISSE, N.; CABRERA, A.; HERNÁN, J.; COSSE, M.; GIANNITTI, F.; FRANCIA, M.E.; GONZÁLEZ, T.; IRIARTE, A.; RIET-CORREA, F.; ROBELLO, C.; GONZÁLEZ, S. |
|
Afiliación : |
NATALIA MANISSE, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; ANDRÉS CABRERA, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; JUAN HERNÁN, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; MARIANA COSSE, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay.; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA E. FRANCIA, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; TELMA GONZÁLEZ, Departamento de Parasitología y Micología, Facultad de Medicina, Instituto de Higiene, Universidad de La República, Avenida Dr. Alfredo Navarro 3051, 11600, Montevideo, Uruguay.; ANDRÉS IRIARTE, Laboratorio de Biología Computacional, Departamento de Desarrollo Biotecnológico, Instituto de Higiene, Universidad de La República Facultad de Medicina, Avenida Dr. Alfredo Navarro 3051, 11600, Montevideo, Uruguay.; FRANKLIN RIET-CORREA AMARAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay./Programa de Pós-graduação em Ciência Animal nos Trópicos, Universidade Federal da Bahia, Salvador, Bahia, Brazil.; CARLOS ROBELLO, Laboratorio de Interacciones Hospedero- Patógeno, Unidad de Biología Molecular Institut Pasteur de Montevideo, Mataojo 2020, 11400, Montevideo, Uruguay.; SUSANA GONZÁLEZ, Departamento de Biodiversidad y Genética, Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Avenida Italia 3318, 11600, Montevideo, Uruguay. |
|
Título : |
Molecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes: a contribution to their molecular epidemiology. |
|
Fecha de publicación : |
2021 |
|
Fuente / Imprenta : |
Molecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w |
|
DOI : |
10.1007/s11033-021-06492-w |
|
Idioma : |
Inglés |
|
Notas : |
Article history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de
Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026). |
|
Contenido : |
Abstract:
Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife. |
|
Palabras claves : |
Hammondia spp; NEOSPOROSIS; Neotropical canids; PLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL; PLATAFORMA DE SALUD ANIMAL; Real-time PCR; TOXOPLASMOSIS. |
|
Asunto categoría : |
E16 Enfermedades de los animales |
|
Marc : |
LEADER 02795naa a2200349 a 4500
001 1062284
005 2022-06-27
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-021-06492-w$2DOI
100 1 $aMANISSE, N.
245 $aMolecular detection of coccidian Apicomplexa Parasites isolated from wild crab-eating and pampas foxes through novel TaqMan™ probes$ba contribution to their molecular epidemiology.$h[electronic resource]
260 $c2021
500 $aArticle history: Received: 24 November 2020 / Accepted: 11 June 2021. This study obtained fnancial support from Programa de Desarrollo de Ciencias Básicas (PEDECIBA) and Agencia Nacional de Investigación e Innovación (FSSA_X_2014_1_106026).
520 $aAbstract: Neospora caninum, Toxoplasma gondii and Hammondia spp. are coccidian parasites similar in morphology. Molecular techniques are necessary to detect parasite DNA isolated from stool samples in wild canids because they were reported as defnitive hosts of N. caninum life cycle. The objective of this study was to develop a highly sensitive and accurate molecular method for the identifcation of coccidian Apicomplexa parasites in crab-eating fox (Cerdocyon thous) and pampas fox (Lycalopex gymnocercus). Tissue samples from road-killed animals (pampas fox=46, crab-eating fox=55) and feces (pampas fox=84, crab-eating fox=2) were collected, and species were diagnosed through molecular assay. PCR was used for the amplifcation of a fragment of the coccidian Apicomplexa nss-rRNA gene. Additionally, we developed a novel realtime PCR TaqMan? probe approach to detect T. gondii- Hammondia spp. and N. caninum. This is the frst report of N. caninum DNA in pampas fox feces (n=1), thus it was also detected from pampas fox tissues (n=1). Meanwhile, T. gondiiwas found in tissues of pampas (n=1) and crab-eating (n=1) foxes and H. trifttae in one crab-eating fox tissue. Despite the low percentage (2.5%) of positive samples, the molecular method developed in this study proved to be highly sensitive and accurate allowing to conduct an extensive monitoring analysis for these parasites in wildlife.
653 $aHammondia spp
653 $aNEOSPOROSIS
653 $aNeotropical canids
653 $aPLATAFORMA DE INVESTIGACIÓN EN SALUD ANIMAL
653 $aPLATAFORMA DE SALUD ANIMAL
653 $aReal-time PCR
653 $aTOXOPLASMOSIS
700 1 $aCABRERA, A.
700 1 $aHERNÁN, J.
700 1 $aCOSSE, M.
700 1 $aGIANNITTI, F.
700 1 $aFRANCIA, M.E.
700 1 $aGONZÁLEZ, T.
700 1 $aIRIARTE, A.
700 1 $aRIET-CORREA, F.
700 1 $aROBELLO, C.
700 1 $aGONZÁLEZ, S.
773 $tMolecular Biology Reports, Volume 48, Pages 5013–5021, 2021. Doi: https://doi.org/10.1007/s11033-021-06492-w
Descargar
Esconder MarcPresentar Marc Completo |
|
Registro original : |
INIA La Estanzuela (LE) |
|
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
| Expresión de búsqueda válido. Check! |
|
|
